Search results for "T7 phage"

showing 3 items of 3 documents

Evaluation of T7 and lambda phage display systems for survey of autoantibody profiles in cancer patients.

2008

In the current study we attempted to evaluate the suitability of T7 Select 10-3b and lambdaKM8 phage display systems for the identification of antigens eliciting B cell responses in cancer patients and the production of phage-displayed antigen microarrays that could be exploited for the monitoring of autoantibody profiles. Members of 15 tumour-associated antigen (TAA) families were cloned into both phage display vectors and the TAA mini-libraries were immunoscreened with 22 melanoma patients' sera resulting in the detection of reactivity against members of 5 antigen families in both systems, yet with variable sensitivity. T7 phage display system showed greater sensitivity for the detection …

Melanoma-associated antigenPhage displayMicroarrayT7 phageAntibodies NeoplasmImmunologyAutoantibodyProtein Array AnalysisBiologybiology.organism_classificationVirologyMolecular biologyBacteriophage lambdaBacteriophageAntigenAntigens NeoplasmPeptide LibraryBacteriophage T7Immunology and AllergyHumansGenomic libraryCloning MolecularMelanomaAutoantibodiesGene LibraryJournal of immunological methods
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In vivo phage display: identification of organ-specific peptides using deep sequencing and differential profiling across tissues.

2021

Abstract In vivo phage display is widely used for identification of organ- or disease-specific homing peptides. However, the current in vivo phage biopanning approaches fail to assess biodistribution of specific peptide phages across tissues during the screen, thus necessitating laborious and time-consuming post-screening validation studies on individual peptide phages. Here, we adopted bioinformatics tools used for RNA sequencing for analysis of high-throughput sequencing (HTS) data to estimate the representation of individual peptides during biopanning in vivo. The data from in vivo phage screen were analyzed using differential binding—relative representation of each peptide in the target…

Phage displayT7 phageAcademicSubjects/SCI00010virusesPeptideBiopanningComputational biologyDeep sequencing03 medical and health sciencesMiceIn vivoPeptide LibraryGeneticsAnimalsTissue DistributionMolecular Biology030304 developmental biologychemistry.chemical_classification0303 health sciencesMice Inbred BALB Cbiology030302 biochemistry & molecular biologyRNAHigh-Throughput Nucleotide Sequencingbiology.organism_classificationHigh-Throughput Screening AssayschemistryCell Surface Display TechniquesPeptidesHoming (hematopoietic)Nucleic acids research
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In vivophage display: identification of organ-specific peptides using deep sequencing and differential profiling across tissues

2020

ABSTRACTIn vivophage display is widely used for identification of organ- or disease-specific homing peptides. However, the currentin vivophage biopanning approaches fail to assess biodistribution of specific peptide phages across tissues during the screen, thus necessitating laborious and time-consuming post-screening validation studies on individual peptide phages. Here, we adopted bioinformatics tools used for RNA sequencing for analysis of high throughput sequencing (HTS) data to estimate the representation of individual peptides during biopanningin vivo. The data fromin vivophage screen were analyzed using differential binding – relative representation of each peptide in the target orga…

chemistry.chemical_classificationPhage displaybiologychemistryT7 phageIn vivoPeptideBiopanningComputational biologybiology.organism_classificationDeep sequencingDNA sequencingHoming (hematopoietic)
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